Regulatory

Part:BBa_K4065000:Design

Designed by: Raúl Acosta Murillo   Group: iGEM21_FCB-UANL   (2021-08-14)


Sigma H sensible promotor pSpoIIA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 13
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 13
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Utilization as a regulated promotor in B. subtilis.


Source

Fujita M, Gonzalez-Pastor JE, Losick R. High, and low threshold genes in the Spo0A regulon of Bacillus subtilis. J Bacteriol. 2005; 187(4): 1357-68.

References